An Evaluation of Prepreparation Storage of Frozen Zoological Specimens
Biological specimens for museum collections are acquired because they are important representatives of a species or are critical for ongoing research at an institution. These specimens must be stored in a frozen state until they can be prepared, but the rate of specimen degradation varies under different freezer conditions. If a specimen degrades too much, the types of preparation become limited and the overall quality of the prepared specimen declines. Our objective was to examine the efficacy of various techniques to preserve frozen zoological specimens. To test this, we wrapped chicken wings using various methods and stored them in five different types of freezers. We monitored the mass of the chicken wings over 8 months and documented conditions in each freezer (temperature, relative humidity, door opening frequency and duration). We found desiccation to be the main reason for mass loss from unwrapped specimens, those stored in warmer freezers, and as time in the freezer increased. Therefore, zoological specimens should be prepared soon after being acquired, and stored in plastic bags in the coldest freezer available to minimize desiccation and ensure the ability to prepare them using a variety of preparation methods.Abstract

Pictures of different wrapping treatments of specimens. Specimens wrapped in a bag with no paper towel (A), vacuum sealed with no paper towel (B), no bag and no paper towel (C), bag with paper towel (D), vacuum sealed with paper towel (E).

Predicted mass lost from specimens wrapped in plastic and unwrapped. The predictive model also included the average value for the average daily door opening frequency, average value for relative humidity, no paper towel, and temperature set to −20°C. Error bars are 95% confidence intervals.

Predicted relationship between percent decline in mass of unwrapped specimens and temperature over 8 months. The predictive model also included the average values for average daily door opening frequency and relative humidity. Dashed lines are 95% confidence intervals and crosses are the raw data points.

Predicted mass lost from unwrapped specimens as a function of average door openings per day. The predictive model also included temperature at −20°C and relative humidity at 70%. Dashed lines are 95% confidence intervals.

Predicted cumulative mass lost from unwrapped specimens in five freezers as a function of time.
Contributor Notes
Associate Editor.—Mariana di Giacomo